pISSN 1738-3544eISSN 2288-1662
pISSN 1738-3544eISSN 2288-1662
pISSN 1738-3544Detail of staining procedure from each tissue component
| Harris hematoxylin and eosin stain | Mayer hematoxylin and eosin stain | 5-min modified Mayer hematoxylin and eosin stain | |
|---|---|---|---|
| Hematoxylin | 10 min | 1 min | 5 min |
| Ammonia water | Bluing buffer 1 min after 1 min wash |
Bluing buffer 1 min after 1 min wash |
|
| 0.1% hydrochloric acid | 0.1% hydrochloric acid 2 times dipping after 5 min wash | ||
| Eosin | 30 s | 3 min 20 s | 3 min 20 s |
| Total time | 15 min 35 s | 6 min 20 s | 10 min 20 s |
