pISSN 1738-3544eISSN 2288-1662
pISSN 1738-3544eISSN 2288-1662
pISSN 1738-3544Comparison of the characteristics for qPCR and dPCR
| qPCR | dPCR | |
|---|---|---|
| Quantitation | Relative | Absolute |
| Standard curve | Require | No |
| Reference | Require | No criteria |
| PCR reaction | Bulk sample (flexible response volume) | Sample partitioning (droplet individual PCR) |
| Precision | Middle | High |
| Sensitivity (%) | 0.1∼5 | 0.01∼1 |
| Inhibitors | Vulnerable | High tolerance |
| Data collected | Exponential phase | Based on Poisson statistics in end point |
| Measurement of amplification | Each cycle | End point |
| Copy number variation | Unsuitable | Suitable |
| Dynamic range | High | Middle |
| Reproducibility | Middle | High |
| Throughout | Wide | Many |
| Rare target | Impact | No impact |
| Large volume sample | Suitable | Unsuitable |
| Multiplexing | Low | High |
| Ease of use | Require | Little training |
| Cost | Middle | High |
Abbreviations: qPCR, quantitative polymerase chain reaction; dPCR, digital polymerase chain reaction.
