The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544 pISSN 1738-3544
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Table 5.

Interfering effects of HEPES buffer concentration on the endotoxin test

Method Gel-clot method Chromogenic method
EndosafeⓇ LAL cartridge EndosafeⓇ rCR cartridge
Sample solution PPC Endotoxin value (EU/mL) Spike recovery (%) Endotoxin value (EU/mL) Test suitability Spike recovery (%) Endotoxin value (EU/mL) Test suitability
20 μg/mL ‒ + <0.125 104.0±1.4 <0.500 Pass 143.0±9.8 <0.500 Pass
200 μg/mL ‒ + <0.125 94.5±2.1 <0.500 Pass 158.0±1.4 <0.500 Pass
2,000 μg/mL ‒ + <0.125 94.0±1.4 <0.500 Pass 130.0±2.8 <0.500 Pass

Abbreviations: HEPES, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid; LAL, Limulus Amebocyte Lysate; rCR, recombinant cascade reagent; PPC, positive product control; EU, endotoxin unit; +, firm gel formed; ‒, no gel formed.
Korean J Clin Lab Sci 2024;56:171-80 https://doi.org/10.15324/kjcls.2024.56.2.171
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