Spearman correlation, agreement and ICC analysis between the Neubauer chamber and three manual microscopic examinations for RBC and WBC
| Method | Neubauer cell counting chamber | |||||
|---|---|---|---|---|---|---|
| r | Agreement rate (N=201), N (%) | ICC | ||||
| Same grade | Within ±1 grade | ICC (3.1) | 95% CI | |||
| Novel protocol | ||||||
| RBC | 0.982 |
173 (86.1) | 200 (99.5) | 0.990 |
0.986∼0.993 | |
| WBC | 0.982 |
178 (88.6) | 200 (99.5) | 0.989 |
0.985∼0.992 | |
| S & M labs (1 drop) | ||||||
| RBC | 0.876 |
24 (11.9) | 135 (67.2) | 0.561 |
‒0.216∼0.820 | |
| WBC | 0.876 |
27 (13.4) | 149 (74.1) | 0.492 |
‒0.218∼0.777 | |
| S & M labs (0.015 mL) | ||||||
| RBC | 0.966 |
139 (69.2) | 201 (100) | 0.939 |
0.710∼0.976 | |
| WBC | 0.969 |
144 (71.6) | 201 (100) | 0.914 |
0.555∼0.967 | |
| KAEQAS | ||||||
| RBC | 0.980 |
161 (80.1) | 201 (100) | 0.973 |
0.872∼0.989 | |
| WBC | 0.986 |
171 (85.1) | 201 (100) | 0.968 |
0.839∼0.987 | |
*
Abbreviations: ICC, intraclass correlation coefficient; RBC, red blood cell; WBC, white blood cell; S & M labs, smaller or medium-sized laboratory; KAEQAS, Korean Association of External Quality Assessment Service; r, Spearman correlation coefficient; 95% CI, 95% confidence interval.
