Nucleotide sequence of real-time reverse transcriptase polymerase chain reaction primers for genes
| Gene description |
Direction | Nucleotide sequence (5'-3') |
|---|---|---|
| 16s rRNA | Forward | CCTACGGGAGGCAGCAGTAG |
| Reverse | CAACAGAGCTTTACGATCCGAAA | |
| SMU.1004 (gtfB) | Forward | AGCAATGCAGCCAATCTACAAAT |
| Reverse | ACGAACTTTGCCGTTATTGTCA | |
| SMU.1005 (gtfC) | Forward | GGTTTAACGTCAAAATTAGCTGTATTAGC |
| Reverse | CTCAACCAACCACTGTT | |
| SMU.910 (gtfD) | Forward | ACAGCAGACAGCAGCCAAGA |
| Reverse | ACTGGGTTTGCTGCGTTTG | |
| SMU.2028 (ftf) | Forward | AAATATCAAGGCGGCTACAACG |
| Reverse | CTTCACCAGTCTTAGCATCCTGAA |
a)Open reading frame, based on the genome annotation of Streptococcus mutans provided by The Institute for Genomic Research.
Abbreviations: gtf, glucosyl transferase; ftf, fructosyl transferase.
