
eISSN 2288-1662
LABTypeTM sequence specific oligonucleotide polymerase chain reaction program
Step | Temperature (℃), incubation time | No. of cycles |
---|---|---|
Step1 | 96, 03:00 | 1 |
Step2 | 96, 00:20 | 5 |
60, 00:20 | ||
72, 00:20 | ||
Step3 | 96, 00:10 | 30 |
60, 00:15 | ||
72, 00:20 | ||
Step4 | 72, 10:00 | 1 |
Step5 | 4, forever | 1 |