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Cytological Study on the Cause of the Osteoporotic Side Effects of Adefovir Dipivoxil
Korean J Clin Lab Sci 2019;51:379-385  
Published on September 30, 2019
Copyright © 2019 Korean Society for Clinical Laboratory Science.

Ho Park

Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, Korea
Correspondence to: * Ho Park
Department of Clinical Laboratory Science, Wonkwang Health Science University, 514 Iksan-daero, Iksan 54538, Korea
E-mail: bacoya@naver.com
* ORCID: https://orcid.org/0000-0003-3528-5643
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Osteoporosis is a disease that increases the risk of fractures by inducing a decrease in bone strength by the changes in hormones and a decrease in minerals. Recent reports have indicated that the long-term administration of Adefovir dipivoxil (ADV), which is used as a treatment for the hepatitis virus and AIDS, may have osteoporotic side effects. On the other hand, there are few studies on the cytopathic correlation of these causes. In this study, the biological relevance of ADV was evaluated using osteoblast hFOB1.19 and vascular endothelial cell HUVEC. First, the cells were treated with ADV at different concentrations, and DAPI and crystal violet staining were performed for morphological analysis of each cell and nucleus. A CCK-8 assay, real-time PCR, alkaline phosphatase (ALP) staining, and activity was performed to evaluate the drug effects on cell proliferation, gene expression, and osteoblast differentiation. As a result, ADV induced cell hypertrophy in hFOB1.19 cells and HUVEC cells. Furthermore, ADV not only inhibited cell proliferation and TGF-β expression but was also involved in osteoblast differentiation. Overall, these results provide basic data to help better understand the mechanism of ADV-induced osteoporosis and its clinical implications.
Keywords : Adefovir dipivoxil, Hypertrophy, Osteogenic differentiation, Osteoporosis, TGF-β

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  • Wonkwang Health Science University