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Effect of Prostaglandin F2 Alpha on E-cadherin, N-cadherin and Cell Adhesion in Ovarian Luteal Theca Cells
Korean J Clin Lab Sci 2019;51:360-369  
Published on September 30, 2019
Copyright © 2019 Korean Society for Clinical Laboratory Science.

Sang-Hee Lee1, Bae Dong Jung2, Seunghyung Lee3

1Discipline of ICT, School of Technology, Environments and Design, University of Tasmania, Hobart, Australia
2College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea
3College of Animal Life Sciences, Kangwon National University, Chuncheon, Korea
Correspondence to: * Seunghyung Lee
College of Animal Life Sciences, Kangwon National University, 1 Gangwondeahak-gil, Chuncheon 24341, Korea
E-mail: s.lee@kangwon.ac.kr
* ORCID: https://orcid.org/0000-0001-5862-3663
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Cadherins are essential transmembrane proteins that promote cell-cell adhesion and maintain the corpus luteum structure in the ovary. This study examined the influence of prostaglandin F2 alpha (PGF2α) on E-cadherin, N-cadherin, and adhesion in luteal theca cells (LTCs). The luteal cells were isolated from the mid-phase corpus luteum, and the LTCs were cultured separately from the luteal heterogeneous cells according to the morphology of the mesenchymal cells and to determine if steroidogenic and endothelial cells of LTCs, 3beta-hydroxysteroid dehydrogenase (3β-HSD), and vascular endothelial growth factor receptor 2 (VEGFR2) mRNA were used. The LTCs were then incubated in the culture medium supplemented with 0.01, 0.1, and 1.0 mM PGF2α for 24 h, and the E-cadherin and N-cadherin proteins in the LTCs were detected by confocal laser scanning microscopy. The results revealed 3β-HSD mRNA expression in the LTC but no VEGF2R mRNA expression. The E-cadherin and N-cadherin proteins of the LTCs were damaged in the 0.01, 0.1, and 1.0 mM PGF2α treatment groups, and the expression of the N-cadherin protein was reduced significantly in 0.01 mM PGF2α compared to the 0 mM PGF2α treatment groups (P<0.05). In addition, the number of attached LTCs were significantly lower in the 0.01 mM PGF2α treatment group than in the 0 mM PGF2α treatment group (P<0.05). In conclusion, PGF2α affected the disruption of cadherin proteins and cell adhesion in LTCs. These results may help better understand the cadherin and adhesion mechanism during corpus luteum regression in the ovary.
Keywords : Adhesion, Corpus luteum, E-cadherin, N-cadherin, Prostaglandin F2 alpha

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