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Effect of Evodiae fructus Methanol Extract on Virulence-Related Genes' Expression of Helicobacter pylori
Korean J Clin Lab Sci 2019;51:316-322  
Published on September 30, 2019
Copyright © 2019 Korean Society for Clinical Laboratory Science.

Ji Yeong Yang1, Pyeongjae Lee2, Jong-Bae Kim1

1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea
2School of Industrial Bio-pharmaceutical Science, Semyung University, Jecheon, Korea
Correspondence to: * Jong-Bae Kim
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, 1 Yonseidae-gil, Wonju 26493, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Helicobacter pylori, a gram-negative bacterium, is one of the risk factors that induces gastritis and gastric cancer. Therefore, much attention has been paid to the compounds that inhibit bacterial growth or eradicate bacteria. Evodiae fructus (EF), the fruit of Evodia rutaecarpa, has been used for treating diarrhea and abdominal pain. EF extract was already found to inhibit the growth of H. pylori. However, to the best of our knowledge, the effect of EF on the virulence factors of H. pylori has not been reported. In this study, when comparing the minimum inhibitory concentration (MIC) of the different methanol concentration extracts, the 95% methanol extract (EF95) showed the lowest MIC value. EF95 extract suppressed the expressions of cagA, vacA and ureB, but interestingly, it up-regulated the expression of ureA. A decrease in production of ammonia in the culture medium and the cell lysates indicated that EF95 inhibited the urease activity in H. pylori, which was the result of EF95 inhibiting the ureB expression. Although the mechanism by which EF95 extract regulates the virulence factors in H. pylori needs further study, EF95 could be used for treatment of gastric troubles induced by H. pylori.
Keywords : CagA, Evodiae fructus, Helicobacter pylori, Urease, VacA

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