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Inhibition Effect on Pathogenic Microbes and Antimicrobial Resistance of Probiotics
Korean J Clin Lab Sci 2019;51:294-300  
Published on September 30, 2019
Copyright © 2019 Korean Society for Clinical Laboratory Science.

Jae Soo Kim1,†, Young Sam Yuk2,†, Ga Yeon Kim3

1Department of Laboratory Medicine, Dankook University Hospital, Cheonan, Korea
2Department of Biomedical Laboratory Science, College of Health Sciences, Dankook University, Korea
3Department of Public Health, Dankook University Graduate School, Cheonan, Korea
Correspondence to: * Ga Yeon Kim
Department of Public Health, Dankook University Graduate School, Dandae-ro, Dongnam-gu, Cheonan 3116, Korea
E-mail: sysnhj77@gmail.com
* ORCID: https://orcid.org/0000-0001-8751-5055
These authors contributed equally to this work.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To investigate the inhibition effect on pathogenic microbes and the antimicrobial resistance of probiotics, a total of 140 probiotics were isolated from 35 kinds of Korean commercially available Kimchi. Of those, L. plantarum was identified from 53 strains (37.9%), E. faecium from 27 strains (19.3%), and L. rhamnosus from 7 strains (5.0%) using 16S rRNA gene sequencing. Sixty nine strains (49.3%) showed overall antimicrobial activity against pathogenic microbes, namely S. Typhi, S. Enteritidis, E. coli O157:H7, S. flexneri, NAG Vibrio, Listeria monocytogenesis, Y. enterocolitica, S. aureus, S. pyogenes, G. vaginalis, C. albicans, and P. acne. The proportions of L. plantarum, E. faecium, and L. rhamnosus strains to pathogenic microbes were 75.5%, 40.7%, and 28.6%, respectively. In addition, a resistance test with 18 antimicrobial agents using a disk diffusion assay revealed a resistance incidence of 98.6% for nalidixic acid, 83.6% for streptomycin, 75.7% for gentamicin 73.6% for vancomycin, 72.1% for norfloxacin, and 67.9% for ciprofloxacin. In conclusion, L. plantarum, L. sakei, and E. faecium strains with various antimicrobial activities and broad antibiotic resistance are useful for treating diarrhea in long-term inpatients and for the alternative use for treating Candida species female vaginitis.
Keywords : Antibiotic resistance, Pathogenic microbes, Probiotics

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