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Menadione Induced Apoptosis in MKN45 Cells via Down-regulation of Survivin
Korean J Clin Lab Sci 2019;51:71-77  
Published on March 31, 2019
Copyright © 2019 Korean Society for Clinical Laboratory Science.

Min Ho Lee1,2,†, Jeongyong Kim1,2,†, Yoonjung Cho2, Do Hyun Kim1, Ji Yeong Yang1, Hye Jin Kwon1,Min Park3, Hyun Jun Woo4, Sa-Hyun Kim5, Jong-Bae Kim1

1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea, 2Forensic DNA Division, National Forensic Service, Wonju, Korea, 3Department of Biomedical Laboratory Science, Daekyeung University, Gyeongsan, Korea , 4Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University, Gyeongsan, Korea,  5Department of Clinical Laboratory Science, Semyung University, Jecheon, Korea
Correspondence to: Jong-Bae Kim
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, 1 Yonseidae-gil, Wonju 26493, Korea, Tel: 82-33-760-2423, Fax: 82-33-760-2561, E-mail: kimjb70@yonsei.ac.kr, ORCID: https://orcid.org/0000-0002-6502-2281
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Menadione is known as an anti-tumor factor. Many studies have reported the potential anti-cancer role of menadione against a range of cancer cell lines. In this study, the anti-cancer effects of menadione and the underlying molecular signaling involved in apoptosis was investigated in gastric cancer cell lines. The menadione treatment decreased the cell viability of MKN45 gastric cancer cells. The decreased cell viability was attributed to the induction of apoptosis, which was confirmed by the results indicating the activation of caspase-3 and -7 and the cleavage of PARP in Western blotting. The upstream regulatory molecules involved in apoptosis were investigated further and it was discovered that menadione reduced the expression of survivin, an inhibitor of upstream apoptosis proteins. In addition, a transcription factor β–catenin, which is known to regulate survivin expression, was down-regulated by menadione. A previous report showed that menadione inhibited XIAP expression to induce apoptosis and induced G2/M cell cycle arrest in AGS cells. This study elucidated another inhibitory mechanism of menadione against gastric cancer cells in a different cell line. Although further studies will be needed, the inhibitory mechanism demonstrated in this study will help better understand the anti-cancer effects of menadione.
Keywords : Apoptosis, β–catenin, Menadione, MKN45 cells, Survivin

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