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Alleviating Effects of Euphorbiae humifusae L. Extract on the Neurotoxicity Induced by Lead
Korean J Clin Lab Sci 2018;50:501-510  
Published on December 31, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Sang-Hee Lee1, Young-Mi Seo2

1Department of Internal Medicine, Sanbon Hospital, School of Medicine, Wonkwang University, Gunpo, Korea 2Department of Nursing, College of Medicine, Wonkwang Health Science University, Iksan, Korea
Correspondence to: Young-Mi Seo
Department of Nursing, College of Medicine, Wonkwang Health Science University, 514 Iksandae-ro, Iksan 54538, Korea
Tel: 82-63-840-1314 Fax: 82-63-840-1319 E-mail:
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The XTT50 value of LA was calculated at a concentration of 38.6 M after C6 glioma cells were incubated for 72 hours in the media containing 30∼50 M of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl-radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.
Keywords : Antioxidative effect, Neurotoxicity, Oxidative stress

December 2018, 50 (4)
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