search for   

 

Expression of H-ras, RLIP76 mRNA and Protein, and Angiogenic Receptors in Corpus Luteum Tissues during Estrous Cycles
Korean J Clin Lab Sci 2018;50:457-461  
Published on December 31, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Minseong Kim1, Sang-Hee Lee2, Seunghyung Lee1

1Department of Animal Industry Convergence, College of Animal Life Sciences, Kangwon National University, Chuncheon, Korea 2Institute of Animal Resources, Kangwon National University, Chuncheon, Koera
Correspondence to: Seunghyung Lee
Department of Animal Industry Convergence, College of Animal Life Sciences, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon 24341, Korea
Tel: 82-33-250-8637 Fax: 82-33-259-5572 E-mail: s.lee@kangwon.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Corpus luteum (CL) is a transient endocrinal tissue that undergoes repeated formation and regression during the estrous cycle. In this study, we hypothesized that the functional and structural mechanism of angiogenesis is similar between CL and tumor formation. First, we measured mRNA and protein expression of angiogenic receptors (vascular endothelial growth factor receptor-2, VEGFR2; Tie 2) in the early, middle, and late phase CL tissues during the estrous cycle. Ral-interacting protein of 76 kDa (RLIP76) and H-ras mRNA and protein were also expressed in the CL tissues. VEGFR2 and Tie 2 mRNA and protein were expressed in the early and middle phase CLs and decreased in the late phase. H-ras mRNA and protein were expressed in the early and middle phase CLs, but not in the late phase. RLIP76 mRNA was expressed in all phase CLs, and the protein expression was highest in early phase CLs. We suggest that RLIP76 and H-ras, an oncogenic gene, regulate the function of the CL during the estrous cycle, and the proteins will play an important role in the angiogenic mechanism of the CL.
Keywords : Angiogenesis, Corpus luteum, H-ras, RLIP76

December 2018, 50 (4)
Full Text(PDF) Free

Cited By Articles
  • CrossRef (0)

Funding Information