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Two-year Quaternary Isolation of Gram-positive Bacilli Using MALDI-TOF MS in Positive Blood Culture of a University Hospital
Korean J Clin Lab Sci 2018;50:414-421  
Published on December 31, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Jin-Un Choi1, Young-Bin Yu2, Sang-Ha Kim3, Seungho Won4, Young-Kwon Kim2

1Department of Laboratory Medicine, Chonnam National University Hospital, Gwangju, Korea
2Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon, Korea
3Department of Laboratory Medicine, Konyang University Hospital, Daejeon, Korea
4Department of Mechanical Engineering, Konyang University, Nonsan, Korea
Correspondence to: Young-Kwon Kim
Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, 158 Gwanjeodong-ro, Seo-gu, Daejeon 35365, Korea
Tel: 82-42-600-8431 Fax: 82-42-543-6370 E-mail: ykkim3245@konyang.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In this study, Gram-positive bacilli (GPB) were identified by MALDI-TOF MS and analyzed according to the quaternary and microbial strains in the blood culture medium over a two year period at a university hospital. The results were as follows. The overall positive rate of blood culture was 9.97%. In 713 isolated GPB, 410 strains (57.5%) were identified using a microflex MALDI Biotyper. The positive rate of GPB among the blood culture positive bacteria was 8.2%, and the quarterly isolation rate was 9.8% in the third quarter of 2015, 8.7% in the second quarter of 2016, 8.1% in the third quarter of 2016, 8.1% in the first quarter of 2015, 7.9% in the first quarter of 2015, 7.9% in the second quarter of 2015, 6.8% in the first quarter of 2016, and 6.7% in the fourth quarter of 2015. The isolates were Corynebacterium striatum 89 (12.4%), Bacillus cereus 60 (8.4%), Bacillus subtilis 30 (4.2%), Paenibacillus urinalis 29 (4.1%), and Listeria monocytogenes 25 (3.5%). The results of 16S rRNA sequencing of 43 isolates (86.0%) were consistent with those of the other 50 isolates. Five out of the seven unmatched weeks were not identified by MALDI-TOF MS
Keywords : Blood culture, Gram positive bacilli, Isolation rate, MALDI-TOF MS

December 2018, 50 (4)
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