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Estimation of HCV Test in Diagnosis for Chronic Hepatitis C Virus
Korean J Clin Lab Sci 2018;50:261-266  
Published on September 30, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Soon-Mo Jang, Byoung Seon Yang

Department of Medical Laboratory Science, Jinju Health College, Jinju, Korea
Correspondence to: Byoung Seon Yang
Department of Medical Laboratory Science, Jinju Health College, 51 Uibyeong-ro, 52655 Jinju, Korea
Tel: 82-55-740-1821
Fax: 82-55-743-3010
E-mail: ybseon@jhc.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To determine the clinical utility of an immunoblot test and RT-PCR-hybridization test, 160 samples from patients with a chronic HCV infection were analyzed by two tests. A total of 133 samples out of 150 positive samples were positive by RT-PCR-hybridization. The true positive rate of the immunoblot tests and the concordance rate of the two tests was 88.6% and 89.3%, respectively. Serotyping and genotyping were performed to evaluate the distribution of the HCV subtype in Korean isolates. HCV serotypes 1 and 2, and genotypes 1b and 2a were the most common sources of HCV infections in this group. In 49 cases studied with the serotypes and genotypes, serotypes 1 and 2 were 57.1% and 42.9%, respectively. Genotypes 1b, 1b/2b, 2a, 2a/2c, and 2b were 51.0%, 2.0%, 34.7%, 8.2%, and 4.1%, respectively. This study shows that immunoblot tests are more useful for screening HCV infections. The RT-PCR-hybridization test confirmed the HCV infection in patients with positive immunoblot test results. The serotype test is preferred over the genotype test for monitoring the progression or response to treatment. On the other hand, there were no significant differences in the response to an α-interferon treatment of HCV infection with serotype type 1 or type 2 in Korea.
Keywords : Hepatitis C virus, Immunoblot, RT-PCR-hybridization

September 2018, 50 (3)
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