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Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma
Korean J Clin Lab Sci 2018;50:197-204  
Published on June 30, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Woo-Soon Choi

Department of Clinical Laboratory Science, Songho University, Hoengseong, Korea
Correspondence to: Woo-Soon Choi
Department of Clinical Laboratory Science, Songho University, 210 Namsan-ro, Hoengseong 25242, Korea Tel: 82-33-340-1141 Fax: 82-33-340-1127 E-mail:
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Multiple myeloma (MM) is the leading cause of death among hematologic neoplasms. Recently, microRNA has been reported to be useful in the diagnosis of multiple myeloma. This study examined whether miR-221 could be used as a diagnostic marker for multiple myeloma. The study was performed on 20 patients with multiple myeloma without any other hematological diseases. MicroRNA extraction was performed using formalin-fixed paraffin-embedded (FFPE) tissues obtained from the bone marrow of patients with multiple myeloma. miR-15a, miR-16, miR-21, miR-181a, and miR-221 were selected as the microRNA target genes for multiple myeloma. The significance of microRNA was based on a fold change of <1.5. To quantify the fold changes, data normalized to the human gene, SNORD43, were used as the values of the patient group. Fold change values greater than 1.5 were defined as “overexpression”, whereas values less than -1.5 were defined as “underexpression”. Of note, 65.0% (13/20) of samples showed significant “overexpression” in the levels of miR-221 expression and plasma cells with a group of more and less than 30% in MM patients did not show any significance of plasma cell (P<0.05). The results of other studies showing a correlation between the expression of miR-221 and MM in Caucasians were confirmed. These results suggest that miR-221 may be a useful indicator for diagnosing patients with MM. In conclusion, miR-221 is useful in the diagnosis and determining the prognosis of multiple myeloma in Koreans.
Keywords : Bone marrow, Hematologic neoplasms, MicroRNAs, Multiple myeloma, Plasma cells

June 2018, 50 (2)
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