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Maintenance of Platelet Counts with Low Level QC Materials and the Change in P-LCR according to Hemolysis with XN-9000
Korean J Clin Lab Sci 2018;50:399-405  
Published on December 31, 2018
Copyright © 2018 Korean Society for Clinical Laboratory Science.

Moon-Jung Shim1, Hyun-A Lee2

1Department of Clinical Laboratory Science, Ansan University, Ansan, Korea 2Department of Laboratory Medicine, Korea University Medical Center, Ansan Hospital, Ansan, Korea
Correspondence to: Moon-Jung Shim
Department of Clinical Laboratory Science, Ansan University, 155 Ansandaehak-ro, Sangrok-gu, Ansan 15328, Korea
Tel: 82-31-400-6938 Fax: 82-31-363-7702 E-mail: mjshim@ansan.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical
laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the
platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in
the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.
Keywords : Hemolysis, Platelet large cell ratio, platelet counting with fluorescence, Quality control,XN 9000


December 2018, 50 (4)
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