Primer sequences used for PCR and RT-PCR
| Primer | Sequences (5′→3′) | |
|---|---|---|
| CPV-2 | Forward | 5’-CAG GAA GAT ATC CAG AAG GA-3’ |
| Reverse | 5’-GGT GCT AGT TGA TAT GTA ATA AAC A-3’ | |
| CCoV | cDNA (reverse) | 5’-TCT GTT GAG TAA TCA CCA GCT-3’ |
| Forward | 5’-TCC AGA TAT GTA ATG TTC GG-3’ | |
| Reverse | 5’-TCT GTT GAG TAA TCA CCA GCT-3’ | |
Abbreviations: PCR, polymerase chain reaction; RT-PCR, reverse transcription PCR; CPV-2, canine parvovirus type 2; CCoV, canine coronavirus; cDNA, complementary deoxyribonucleic acid.
