Bacteroides fragilis toxin induces sequential cleavage of E-cad-herin and degradation of the 28-kDa E-cadherin intracellular fragment in BT-474 cells. Human breast epithelial BT-474 cells were pre-treated with (A) γ-secretase inhibitor (L-685,458; 1.5 μM) or proteasome inhibitor, (B) borte-zomib (2 μM) or (C) carfilzomib (2 μM) for 30 min and incubated with BHIB, rNT or rET for indicated time period (10, 30, 60, and 180 minutes). Cell lysates were examined by Western blot analysis using C-terminal specific E-cadherin antibodies. GAPDH was used as internal control. Abbreviations: See
Figure 2.
