Fig. 2. Bacteroides fragilis toxin-induced 28 kDa E-cadherin intracellular fragment is degraded by the proteasome complex in HT29/C1 cells. Colonic epithelial HT29/C1 cells were pre-treated with proteasome inhibitors, (A) bortezomib (2 μM) or (B) carfilzomib (2 μM) for 30 minutes and incubated with BHIB, rNT or rET for indicated time period (10, 30, 60, and 180 minutes). Cell lysates were examined by Western blot analysis using C-terminal specific E-cadherin antibodies. GAPDH was used as internal control. Abbreviations: BHIB, brain heart infusion broth; rNT, rNTBF supernatant; rET, rETBF supernatant; E-cad, E-cadherin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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