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Table. 6.

Table. 6.

Concordance rates between PCR-EGFR, IHC-ALK (D5F3), and FISH-ROS1 and NGS analysis in lung adenocarcinoma cases

Methodology NGS

Positive Negative Total
PCR-EGFR
Positive 40* 0 40
Negative 0 43 43
Total 40 43 83
Concentration rates (%) 100 (κ=1.00)
IHC-ALK (D5F3)
Positive 2 1 3
Negative 0 70 70
Total 2 71 73
Concentration rates (%) 98.6 (κ=0.79)
FISH-ROS1
Positive 3 0 3
Negative 0 74 74
Total 3 77 77
Concentration rates (%) 100 (κ=1.00)

Abbreviations: PCR, polymerase chain reaction; EGFR, epidermal growth factor receptor; NGS, next generation sequencing; IHC, immunohistochemistry; ALK, anaplastic lymphoma kinase; FISH, fluorescence in situ hybridization; ROS1, receptor tyrosine kinase 1.

*In addition to the EGFR mutations, the two cases were accom-panied by mutations at different locations in PCR (case 6). and NGS (case 11); In one case, the EGFR mutation was confirmed in NGS, but it was located in exon 18 and judged to be accordant.

Korean J Clin Lab Sci 2023;55:16-28 https://doi.org/10.15324/kjcls.2023.55.1.16
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