Concordance rates between PCR-EGFR, IHC-ALK (D5F3), and FISH-ROS1 and NGS analysis in lung adenocarcinoma cases
| Methodology | NGS | ||
|---|---|---|---|
|
|
|||
| Positive | Negative | Total | |
| PCR-EGFR | |||
| Positive | 40* | 0 | 40 |
| Negative | 0† | 43 | 43 |
| Total | 40 | 43 | 83 |
| Concentration rates (%) | 100 (κ=1.00) | ||
| IHC-ALK (D5F3) | |||
| Positive | 2 | 1 | 3 |
| Negative | 0 | 70 | 70 |
| Total | 2 | 71 | 73 |
| Concentration rates (%) | 98.6 (κ=0.79) | ||
| FISH-ROS1 | |||
| Positive | 3 | 0 | 3 |
| Negative | 0 | 74 | 74 |
| Total | 3 | 77 | 77 |
| Concentration rates (%) | 100 (κ=1.00) | ||
Abbreviations: PCR, polymerase chain reaction; EGFR, epidermal growth factor receptor; NGS, next generation sequencing; IHC, immunohistochemistry; ALK, anaplastic lymphoma kinase; FISH, fluorescence in situ hybridization; ROS1, receptor tyrosine kinase 1.
*In addition to the EGFR mutations, the two cases were accom-panied by mutations at different locations in PCR (case 6). and NGS (case 11); †In one case, the EGFR mutation was confirmed in NGS, but it was located in exon 18 and judged to be accordant.
