
Representation of selected primer pairs and fragments generated after digestion with specific restriction enzymes
Genes | SNPs | Primer pairs | Size | Enzyme | Fragmented size |
---|---|---|---|---|---|
rs1799998 | 5’-GACTCCAGGACCCTGGTTGATA-3’ 5’-CAGCCAAAGGTAGATGAAGGAG-3’ |
390 | SFoI | 390, 204, 186 | |
rs4646903 | 5’-CAGTGAAAGAGGTGTAGCCGCT-3’ 5’-TAGGGAGTCTTGTCTCATGCCT-3’ |
340 | MSPI | 340, 205, 135 |