Fig. 3. Melting curve analysis of carbapenemase gene. (A) melting curve analysis of blaKPC amplicon was generated that showed the fragment melting temperature (Tm) 87.5°C. (B) melting curve analysis of blaNDM amplicon was generated that showed the fragment Tm 88°C. (C) melting curve analysis of blaIMP amplicon was generated that showed the fragment Tm 81°C. (D) melting curve analysis of blaVIM amplicon was generated that showed the fragment Tm 88.5°C. (E) melting curve analysis of blaOXA-48-like amplicon was generated that showed the fragment Tm 79°C.
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