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Table. 2.

Table. 2.

Real time PCR conditions and melt curve program for diagnosis of flaviviruses

Cycle Step Temperature Time
1 cycle Reverse transcription 50°C 15 min
Enzyme activation 95°C 10 min
40 cycles Denaturation 95°C 10 sec
Annealing 60°C 15 sec
Extension 72°C 10 sec
Signal reading 80°C 30 sec
1 cycle Denaturation 95°C 30 sec
1 cycle Melting* 60∼95°C 15 sec
Cooling 25°C 30 min

*Increase in temperature set at 0.5°C per cycle.

Korean J Clin Lab Sci 2022;54:142-8 https://doi.org/10.15324/kjcls.2022.54.2.142
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