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Table. 6.

Table. 6.

Comparison of RT-qPCR and serological immunosaaay

RT-qPCR test Serological antibody test
Advantage High specific Easy to use serological sample
Restriction

Sensitivity can decrease due to specimen sampling errors or inadequate viral load (false negative).

Inactive virus and viral fragments could also test positive (false positive).

Not as accurate as RT-qPCR test, with false positive and false negative.

False positive in a low prevalence population can give an overstatement of exposure and immunity.

Put right Testing twice sequentially to enhance sensitivity. Analysis validation with sufficient positive and negative sample cohorts; generally cannot be used to diagnosis test newly infected patients, but can be used as a screening test
Major utility Standard of care diagnosis of newly infected and/or active COVID-19 patients. Screening test for stratifying newly infected patients, surveillance assay for seroprevalence, immunity and vaccination efficacy.

Abbreviation: RT-qPCR, reverse transcription quantitative polymerase chain reaction.

References [8, 14].

Korean J Clin Lab Sci 2022;54:79-94 https://doi.org/10.15324/kjcls.2022.54.2.79
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