The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Conditions for nested-PCR

Amplification conditions Temperature Time Cycle
1st PCR Initial denaturation 94°C 5 min 1 cycle
Denaturation 94°C 30 sec 40 cycle
Annealing 58°C 30 sec
Extension 72°C 40 sec
Final extension 72°C 5 min 1 cycle
2nd PCR Initial denaturation 94°C 5 min 1 cycle
Denaturation 94°C 30 sec 35 cycle
Annealing 58°C 30 sec
Extension 72°C 40 sec
Final extension 72°C 5 min 1 cycle
Korean J Clin Lab Sci 2021;53:317-25 https://doi.org/10.15324/kjcls.2021.53.4.317
© 2021 Korean J Clin Lab Sci