The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Fig. 1.

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Fig. 1. Caspase-8 activates effector caspases and cleaves PAPR in TG-accumulated THP-1 macrophages. THP-1 cells were differen-tiated into macrophages by treatment of 200 nM PMA for 48 h. The differentiated THP-1 macrophages were incubated with TG in the absence or presence of the caspase-8 inhibitor, Z-IETD-fmk for additional 24 h. (A) Activation of caspase-8 was detected by Western blotting. β-actin was used as an internal control. (B) Viable cells were enumerated by the trypan blue dye exclusion assay. The number of viable cells in THP-1 macrophages without TG treatment was set as 100%. (C) Activation of caspase-3 and (D) cleaved form of PARP was detected by Western blotting. All data are expressed as the mean±SEM of three independent experiments. P-values were determined with Student’s t-test. **P<0.01, ***P<0.001.
Korean J Clin Lab Sci 2021;53:158-64
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