The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Biosafety level and safety equipment according to each risk group

BSL Practices Safety equipment
(primary barriers)
(secondary barriers)
1 Basic – BSL 1 Standard microbiological practices No primary barriers required Laboratory bench and sink required
2 Basic – BSL 2 BSL-1 practice plus
- Limited access
- Sharp precautions
Primary barriers
- BSC used for all manipulations of agents of infectious materials
- PPE: gown, gloves, face shield
BSL-1 plus
- Autoclave available
3 Containment– BSL 3 BSL-2 practice plus
- Controlled access
- Decontamination of all waste
Primary barriers
- BSC used for all open manipulations
- PPE: Protective laboratory clothing, gloves, face, mask
BSL-2 plus
- Physical separation from access corridors
- Self-closing, double-door access
- Exhausted air not recirculated
- Negative airflow into laboratory
- Entry through airlock or anteroom
- Hand washing sink near laboratory exit
4 Maximumcontainment– BSL 4 BSL-2 practice plus
- Clothing change before entering
- Shower on exit
- All material deontaminated on exit from facility
BSL-3 plus
- Separate building or isolated zone
- Dedicated supply and exhaust, vacuum, and decontamination systems

Abbreviations: BSC, biological safety cabinet; BSL, biosafety level; NP, negative pressure; PPE, personal protective equipment.

Korean J Clin Lab Sci 2021;53:143-50
© 2021 Korean J Clin Lab Sci