The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 2.

Table. 2.

Precautions and requirements regarding safe use of COVID-19 samples associated with SARS-CoV-2 virus extracted from the MOH Interim Guidelines (MOH Circular 17/2020)

Procedures Precaution and requirement
Virus culture and manipulation of live virus (e.g., virus isolation, virus neutralisation assay, packaging of positive cultures) To be carried out in a certified Biosafety Level 3 (BSL 3) Laboratory with BSL 3 practices
Serology assay (e.g. antigen or antibody detection)or To be carried out in Biosafety Level 2 Plus (BSL 2+) Laboratory
-Standard personal protective clothing including laboratory coat/gown and gloves; and mucosal and respiratory protection (e.g. eye protection and N95 respirator or equivalent), as indicated by risk assessment
-Handling of samples is to be carried out in a certified Class II Biological Safety Cabinet (BSC)
-Prevent aerosolisation of samples
-Use sealed centrifuge cups or rotors that are unloaded in a Class II BSC
-Secondary leak-proof containers must be used to transport or store samples within the laboratory
-Personnel handling such samples must be trained or have demonstrated proficiency in microbiological practices and techniques
-Emergency response procedures in place and staff are to be trained in the procedures
Molecular assay(e.g. PCR or sequencing)or
Pathological examination and processing of formalin-fixed or otherwise inactivated tissues
Sample/material inventory Implement a robust inventory management system to ensure all samples/materials are labelled, accounted for and their movement could be efficiently tracked
Disinfectants The following disinfectants that are effective on SARS-CoV-2 and shall be considered for use:
-Sodium hypochlorite
-Ice-cold acetone
-Ice-cold acetone/methanol mixture (40:60)
-Ethanol (70%)
-Paraformaldehyde
-Glutaraldehyde
Occupational health Practise self-monitoring for fever or any other related symptoms. Personnel presenting symptoms of respiratory infections and/or has reason to believe exposure to the virus shall immediately report supervisor or the medical authorities so that they can receive appropriate medical advice or management. Any adverse incident or accident involving potential or actual exposure to the virus should be reported for evaluation and advice. MOH Biosafety must also be notified as soon as possible, within 24 hours
Biological waste management Implement procedures to meet local requirements
Transport -Triple packaging; no public conveyances for local transports; shipper must be trained
-International Air shipment as category B (UN 3373) for clinical samples: as category A (UN2814) for viral cultures
Korean J Clin Lab Sci 2021;53:68-80 https://doi.org/10.15324/kjcls.2021.53.1.68
© 2021 Korean J Clin Lab Sci