Sequences of oligonucleotide primers and probe for the detection of JAK2 V617F mutation
| Primer | Direction | Length | Sequence | Modification |
|---|---|---|---|---|
| JAK2-F | Forward | 24 bp | 5′- CTTTCTTTGAAGCAGCAAGTATGA -3′ | - |
| JAK2-WR | Reverse wild type allele | 30 bp | 5′ -GTAGTTTTACTTACTCTCGTCTCCACATAC -3′ | - |
| JAK2-MR | Reverse mutant type allele | 30 bp | 5′ - GTAGTTTTACTTACTCTCGTCTCCACATAA -3′ | - |
| JAK2 Probe | Probe | 30 bp | 5’-TGAGCAAGCTTTCTCACAAGCATTTGGTTT-3’ | Hex-BHQ |
