The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

PCR program for the detection of JAK2 V617F, MPL W515L, and CALR type 1 & 2 mutations

PCR steps Temperature (°C) Time Cycle
Pre-heating 50 2 minutes 1
Pre-denaturation 95 10 minutes 1
Denaturation 95 25 seconds 40
Annealing, elongation and detection 59 for JAK2 V617F 58 for MPL W515L 60 for CALR type 1 & 2 25 seconds
30 seconds
20 seconds
Korean J Clin Lab Sci 2021;53:41-8 https://doi.org/10.15324/kjcls.2021.53.1.41
© 2021 Korean J Clin Lab Sci