Sequences of internal control (template, probe and primer)
| Primer | Direction | Length | Sequence | Modification |
|---|---|---|---|---|
| IC-F | Forward | 21 bp | 5′- CCA AGT GTG AGG GCT GAA AAG -3′ | - |
| IC-R | Reverse | 26 bp | 5′ -TGT TGT GGC TGA TGA ACT ATA AAA GG -3′ | - |
| IC-Probe | Probe | 26 bp | 5′ - CCC CAG TCT CTG TCA GCA CTC CCT TC -3′ | Cy5.5-BHQ |
| IC-Template | DNA Template | 80 bp | 5’-CCA AGT GTG AGG GCT GAA AAG AAT GCC CCA GTC TCT GTC AGC ACT CCC TTC TTC CCT TTT ATA GTT CAT CAG CCA CAA CA-3’ | - |
