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Table. 4.

Table. 4.

Comparison of different respiratory virus molecular detection methods

Assay Target Character Sensitivity (%) Specificity (%) References
Influenza A and B virus m PCR based on testing 1033 specimens 92 84 [40]
multiplex h1 (944 bp)
RT-PCR h3 (591 bp)
ha Multiplex RT-PCR detects 19 viruses using Luminex xMAP system on testing 544 specimens 96.4 for A 95.9 for A
91.5 for B 96.7 for B
Avian influenza virus H5N1 m M-Chip low density microarray using oligos on testing 43 isolates 97 100 [41]
real time
RT-PCR
RSV pol l Multiplex PCR detects 7 viruses (influenza A and B, PIV-1-3, RSV and adenovirus etc.) and IFA based on testing 598 specimens (including 123 RSV positives) 100 100 [42]
multiplex
RT-PCR
Rhinovirus 5′-ncr Multiplex RT-PCR detects 19 viruses and second PCR, sequencing performance based on testing 554 specimens 100 91.3 [43]
multiplex
RT-PCR
QuickVue RSV (dipstick immunoassay) glycoprotein, fusion protein Samples (nasopharyngeal aspirate specimens, NUCLISENS easyMag platform [bioMérieux, MA, USA]) and real-time PCR performed on ABI 7300 instrumentation (AgPath-ID One-Step RT-PCR master mix kit [Ambion, TX, USA]) 90.1 98.8 [44]
Nasopharyngeal swabs (FLOQSwabsTM [Copan Flock Technologies, Brescia, Italy])
Korean J Clin Lab Sci 2021;53:11-8 https://doi.org/10.15324/kjcls.2021.53.1.11
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