Comparison of different respiratory virus molecular detection methods
| Assay | Target | Character | Sensitivity (%) | Specificity (%) | References |
|---|---|---|---|---|---|
| Influenza A and B virus | PCR based on testing 1033 specimens | 92 | 84 | [40] | |
| multiplex | |||||
| RT-PCR | h |
||||
| Multiplex RT-PCR detects 19 viruses using Luminex xMAP system on testing 544 specimens | 96.4 for A | 95.9 for A | |||
| 91.5 for B | 96.7 for B | ||||
| Avian influenza virus H5N1 | M-Chip low density microarray using oligos on testing 43 isolates | 97 | 100 | [41] | |
| real time | |||||
| RT-PCR | |||||
| RSV | Multiplex PCR detects 7 viruses (influenza A and B, PIV-1-3, RSV and adenovirus etc.) and IFA based on testing 598 specimens (including 123 RSV positives) | 100 | 100 | [42] | |
| multiplex | |||||
| RT-PCR | |||||
| Rhinovirus | 5′-ncr | Multiplex RT-PCR detects 19 viruses and second PCR, sequencing performance based on testing 554 specimens | 100 | 91.3 | [43] |
| multiplex | |||||
| RT-PCR | |||||
| QuickVueⓇ RSV (dipstick immunoassay) | glycoprotein, fusion protein | Samples (nasopharyngeal aspirate specimens, NUCLISENSⓇ easyMagⓇ platform [bioMérieuxⓇ, MA, USA]) and real-time PCR performed on ABI 7300 instrumentation (AgPath-ID One-Step RT-PCR master mix kit [AmbionⓇ, TX, USA]) | 90.1 | 98.8 | [44] |
| Nasopharyngeal swabs (FLOQSwabsTM [Copan Flock Technologies, Brescia, Italy]) |
