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Table. 6.

Table. 6.

Data interpretation when amplifying genes for screening and confirmatory test, simultaneously

Case 1 Case 2 Case 3 Case 4 Case 5 Case 6 Case 7
IC (HEX) +/− +/− +/− +/− +/− +
E gene (FAM) + +/− +/− +
RdRP gene(Cal Red 610) + + +
N gene(Quasar 670) + + +
ResultInterpretation 2019-nCoV Detected Inconclusive Result* 2019-nCoVNot Detected, sarbecovirus Detected Negative Invalid

Abbreviations: IC, internal control; HEX, Hexachloro-fluorescein; E gene, SARS-CoV-2 envelope; FAM, 5-carboxylfluorescein; RdRP gene, RNA-dependent RNA polymerase; N gene, SARS-CoV-2 nucleocapsid.

1) Recommended to re-test by increasing the sample concentration. 2) Recommended to proceed with sequencing.

Korean J Clin Lab Sci 2020;52:284-95 https://doi.org/10.15324/kjcls.2020.52.3.284
© 2020 Korean J Clin Lab Sci