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Table. 5.

Table. 5.

Data interpretation when amplifying genes for screening and confirmatory test, respectively

Screening test Confirmatory test


PC NC FAM (E) JOE (IC) Interpretation PC NC FAM (RdRp) JOE (IC) Interpretation
1 + + + betaCoV (+) + + + 2019-nCoV (+)
2 + + + +


3 + + betaCoV (−) + + 2019-nCoV (−)


4 + Invalid result/Retest + Invalid result/Retest
5 + + +/‒ +/‒ + + +/‒ +/‒
6 + +/‒ +/‒ + +/‒ +/‒
7 +/‒ +/‒ +/‒ +/‒

Abbreviations: PC, Positive control; NC, Negative control; FAM, 5-carboxylfluorescein; JOE, d 2',7'-dimethoxy-4',5'-dichloro- 6-carboxyfluorescein; IC, internal control; E gene, SARS-CoV-2 envelope; RdRP gene, RNA-dependent RNA polymerase; betaCoV, betacoronavirus; nCoV, novel coronavirus.

Korean J Clin Lab Sci 2020;52:284-95 https://doi.org/10.15324/kjcls.2020.52.3.284
© 2020 Korean J Clin Lab Sci