The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Specific primer sets used in this study for subtyping of SCCmec type & mec complex subtypes in methicillin-resistant Staphylococcus aureus isolate

Target regions Nucleotide sequences (5’-3’) Amplicon size (bp) Accession No. Reference
SCCmec subtypes SCCmec type Ⅰ Forward 5’-GCT TTA AAG AGT GTC GTT ACA GG-3’ 613 AB033763 Zhang et al, 2005 [10]
Reverse 5’-GTT CTC TCA TAG TAT GAC GTC C-3’
SCCmec type Ⅱ Forward 5’-CGT TGA AGA TGA TGA AGC G-3’ 398 D86934
Reverse 5’-CGA AAT CAA TGG TTA ATG GAC C-3’
SCCmec type Ⅲ Forward 5’-CCA TAT TGT GTA CGA TGC G-3’ 280 AB37671
Reverse 5’-CCT TAG TTG TCG TAA CAG ATC G-3’
SCCmec type Ⅳa Forward 5’-GCC TTA TTC GAA GAA ACC G-3’ 776 AB063172
Reverse 5’-CTA CTC TTC TGA AAA GCG TCG-3’
SCCmec type Ⅳb Forward 5’-TCT GGA ATT ACT TCA GCT GC-3’ 493 AB063173
Reverse 5’-AAA CAA TAT TGC TCT CCC TC-3’
SCCmec type Ⅳc Forward 5’-ACA ATA TTT GTA TTA TCG GAG AGC-3’ 200 AB096217
Reverse 5’-TTG GTA TGA GGT ATT GCT GG-3’
SCCmec type Ⅳd Forward 5’-CTC AAA ATA CGG ACC CCA ATA CA-3’ 881 AB097677
Reverse 5’-TGC TCC AGT AAT TGC TAA AG-3’
SCCmec type Ⅴ Forward 5’-GAA CAT TGT TAC TTA AAT GAG CG-3’ 325 AB12121
Reverse 5’-TGA AAG TTG TAC CCT TGA CAC C-3’
Methicillin resistance mecA gene Forward 5’-GTG AAG ATA TAC CAA GTG ATT-3’ 147 X52593
Reverse 5’-ATG CGC TAT AGA TTG AAA GGA T-3’
mec complex subtypes mec complex group A Forward 5’-CCC TTT TTA TAC AAT CTC GTT-3’ 146 IS1272
Reverse 5’-ATA TCA TCT GCA GAA TGG G-3’
mec complex group B Forward 5’-TAT TTT TGG GTT TCA CTC GG-3’ 1,305 CA05
Reverse 5’-CTC CAC GTT AAT TCC ATT AAT ACC-3’
Korean J Clin Lab Sci 2020;52:128-35 https://doi.org/10.15324/kjcls.2020.52.2.128
© 2020 Korean J Clin Lab Sci