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Table. 2.

Table. 2.

Multiplex PCR conditions for SCCmec & mec complex subtyping

Targets Pre-denaturation Denaturation Annealing Extension Final extension



1 cycle 40 cycle 1 cycle
mecA 94°C/5 min 94°C/30 sec 50°C/30 sec 72°C/30 sec 72°C/10 min
SCCmec type I 55°C/30 sec 72°C/30 sec
SCCmec type II
SCCmec type III
SCCmec type IVa
SCCmec type IVb 50°C/30 sec 72°C/1 min
SCCmec type IVc 55°C/30 sec 72°C/30 sec
SCCmec type IVd
SCCmec type V
mec complex A 54°C/30 sec 72°C/1 min
mec complex B
Korean J Clin Lab Sci 2020;52:128-35 https://doi.org/10.15324/kjcls.2020.52.2.128
© 2020 Korean J Clin Lab Sci