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Table. 2.

Table. 2.

Development and reliability of pregnancy test using Immunoassay

Analytical techniqueYearTime required (min)Accuracy (%)Sensitivity (IU of hCG/L)Remarks
Hemagglutination inhibition test (Wide-Gemzell test)1960~197390~12098.4~99.6750~1,000 (Compared with bioassay)Using hCG antibody (1960)
Latex agglutination inhibition test1962~19742~391~1001,000~3,500 (Based on product insert papers)
Radioimmunoassay1966~198360~2,16094~99.20.5~200- Initially, hCG measurement (1966) - β-hCG measurement, Overcome the cross- reactivity problem between LH and hCG (1972) - β-hCG uses monoclonal antibodies instead of polyclonal antibodies (1982)
Enzyme immunoassay1982~199010~120-2.7~50
Chemiluminescent enzyme immunometric assay1995~200710~30-0.5~1.2Sensitivity improved by 2 to 10 times than RIA and colorimetric-EIA

Abbreviations: hCG, human chorionic gonadotropin; LH, luteinizing hormone; RIA, radio immunoassay; EIA, enzyme immunoassay.

Korean J Clin Lab Sci 2018;50:382-90
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