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Table. 2.

Table. 2.

Development and reliability of pregnancy test using Immunoassay

Analytical technique Year Time required (min) Accuracy (%) Sensitivity (IU of hCG/L) Remarks
Hemagglutination inhibition test (Wide-Gemzell test) 1960~1973 90~120 98.4~99.6 750~1,000 (Compared with bioassay) Using hCG antibody (1960)
Latex agglutination inhibition test 1962~1974 2~3 91~100 1,000~3,500 (Based on product insert papers)
Radioimmunoassay 1966~1983 60~2,160 94~99.2 0.5~200 - Initially, hCG measurement (1966) - β-hCG measurement, Overcome the cross- reactivity problem between LH and hCG (1972) - β-hCG uses monoclonal antibodies instead of polyclonal antibodies (1982)
Enzyme immunoassay 1982~1990 10~120 - 2.7~50
Chemiluminescent enzyme immunometric assay 1995~2007 10~30 - 0.5~1.2 Sensitivity improved by 2 to 10 times than RIA and colorimetric-EIA

Abbreviations: hCG, human chorionic gonadotropin; LH, luteinizing hormone; RIA, radio immunoassay; EIA, enzyme immunoassay.

Korean J Clin Lab Sci 2018;50:382-90 https://doi.org/10.15324/kjcls.2018.50.4.382
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