The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Development and reliability of pregnancy test using bioassay

Test name Start year Test method Interpretation Accuracy (%) Sensitivity (IU of hCG/L)
Aschheim-zondek test (A-Z test) 1927 A pregnant woman’s urine was subcutaneously injected into an immature female rat or mouse. Observation and dissection after 100 hours. In the case of pregnancy, the mouse would show an estrous reaction (be in heat) and great hypertrophy of the uterine horns, luteinization in the ovary and hemorrhagic follicles were also observed. 98 (average) 3,000~5,000
Friedman test 1929 Injection a pregnant woman’s urine into a female rabbit’s ear vein. Dissection after 48 hours. Confirm ovulation and corpora hemorrhagic formation. 100, Similar accuracy to immature mice 100~1,800
Hogben test (Bufo test) 1930 A pregnant woman’s urine or serum was subcutaneously injected into a female frog. Confirm ovulation within 24 hours. 99.3
Galli-Mainini test 1947 Injection a small amount of urine from a woman who suspects she is pregnant into the dorsal lymph sack of a male frog. Microscopic examination of spermatozoa in urine within 3 hours 94~100

Abbreviation: hCG, human chorionic gonadotropin.

Korean J Clin Lab Sci 2018;50:382-90
© 2018 Korean J Clin Lab Sci