The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Comparison of technical details between conventional cytogenetic and major molecular approaches

Techniques Method Characteristics Application Advantages
Conventional cytogenetics (G-banding, R-banding) Cell culturing Special dye generate banding pattern for each chromosome Detection of numerical and structural chromosomal anomalies Genome wide screening for chromosome level abnormalities
Conventional FISH Molecular technique Labeled DNA is used as a probe to search for target sequences in chromosome Detect all types of balance and unbalanced defects Interphase cytogenetics possible
Simple procedures
Spectral karyotyping Arresting cell in metaphase Chromosome specific probes allows the painting of every chromosome Detection of rearrangements including complex anomalies Fast characterization of euchromatic marker chromosome content
CGH Molecular technique Comparative hybridization of differentially labeled total genomic tumor DNA and reference DNA Identify and assess biomarkers Whole genome wide screening of genomic anomalies
Gene discovery, functional analysis No need for cell culture
Array-CGH Molecular technique Identification of DNA sequences by specific DNA binding proteins in cells Identification of cryptic rearrangements (aneuploidy, deletions, duplications or amplifications) High-resolution target-specific detection of gene amplification, submicroscopic information on imbalances

Abbreviations: FISH, fluorescence in situ hybridization; CGH, Comparative genomic hybridization.

Korean J Clin Lab Sci 2018;50:375-81 https://doi.org/10.15324/kjcls.2018.50.4.375
© 2018 Korean J Clin Lab Sci