The Korean Journal of Clinical Laboratory Science : eISSN 2288-1662 / pISSN 1738-3544

Table. 1.

Table. 1.

Comparison of technical details between conventional cytogenetic and major molecular approaches

TechniquesMethodCharacteristicsApplicationAdvantages
Conventional cytogenetics (G-banding, R-banding)Cell culturingSpecial dye generate banding pattern for each chromosomeDetection of numerical and structural chromosomal anomaliesGenome wide screening for chromosome level abnormalities
Conventional FISHMolecular techniqueLabeled DNA is used as a probe to search for target sequences in chromosomeDetect all types of balance and unbalanced defectsInterphase cytogenetics possible
Simple procedures
Spectral karyotypingArresting cell in metaphaseChromosome specific probes allows the painting of every chromosomeDetection of rearrangements including complex anomaliesFast characterization of euchromatic marker chromosome content
CGHMolecular techniqueComparative hybridization of differentially labeled total genomic tumor DNA and reference DNAIdentify and assess biomarkersWhole genome wide screening of genomic anomalies
Gene discovery, functional analysisNo need for cell culture
Array-CGHMolecular techniqueIdentification of DNA sequences by specific DNA binding proteins in cellsIdentification of cryptic rearrangements (aneuploidy, deletions, duplications or amplifications)High-resolution target-specific detection of gene amplification, submicroscopic information on imbalances

Abbreviations: FISH, fluorescence in situ hybridization; CGH, Comparative genomic hybridization.

Korean J Clin Lab Sci 2018;50:375-81 https://doi.org/10.15324/kjcls.2018.50.4.375
© 2018 Korean J Clin Lab Sci