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Table. 2.

Table. 2.

Overview of guidelines on microbial surveillance of endoscopes

 Sampling techniqueSampling volume, (mL)Volume used for cultureCulture mediumIncubation temperature(℃)Duration of incubation(d)Criterion of acceptance
Flushing with sterile saline20 per channel20 mL---Unclear
Flushing with sterile saline+brush20 per channel20 mL---<20 CFU/channel
Flushing with sterile tensioactive fluid100∼200100∼200 mLNon-selective agar305<25 CFU; no indicator MO
Flushing with sterile saline20 per channel1 mLNon-selective agar302<20 CFU; no indicator MO
Flushing with sterile water or saline+brush10 per channel100 μL (after centrifuagtion)2 blood agars28 357<10 CFU; no indicator MO
Flushing with sterile water+brush10100 μLBlood agar, Sabouraud agar37 302 5<20 CFU/0.1 mL
Flushing with sterile saline+brush-----No vegetative bacteria

Abbreviations: MO, microorganisms; CFU, colony-forming units; -, not mentioned. Sources: references 28 and 32.

Korean J Clin Lab Sci 2018;50:359-69 https://doi.org/10.15324/kjcls.2018.50.3.359
© 2018 Korean J Clin Lab Sci